Paper, Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research
Many applications in plant biology requires editing genomes accurately including correcting point mutations, incorporation of single-nucleotide polymorphisms (SNPs), and introduction of multinucleotide insertion/deletions (indels) into a predetermined position in the genome. These types of modifications are possible using existing genome-editing technologies such as the CRISPR-Cas systems, which require induction of double-stranded breaks in the target DNA site and the supply of a donor DNA molecule that contains the desired edit sequence. However, low frequency of homologous recombination in plants and difficulty of delivering the donor DNA molecules make this process extremely inefficient. Another kind of technology known as base editing can perform precise editing; however, only certain types of modifications can be obtained, e.g., C/G-to-T/A and A/T-to-G/C. Recently, a new type of genome-editing technology, referred to as “prime editing,” has been developed, which can achieve various types of editing such as any base-to-base conversion, including both transitions (C→T, G→A, A→G, and T→C) and transversion mutations (C→A, C→G, G→C, G→T, A→C, A→T, T→A, and T→G), as well as small indels without the requirement for inducing double-stranded break in the DNA. Because prime editing has wide flexibility to achieve different types of edits in the genome, it holds a great potential for developing superior crops for various purposes, such as increasing yield, providing resistance to various abiotic and biotic stresses, and improving quality of plant product. In this review, we describe the prime editing technology and discuss its limitations and potential applications in plant biology research.
Learn about our two Decals!
Click here to find out more about our Fall Bioinspired Design Decal and our Spring Bioinspired Design in Action Decal – ALL MAJORS are welcome.
Berkeley BioDesign Community
Click here to learn about the BioD: Bio-Inspired Design @ Berkeley student organization or here to signup for more info.
Search
Student Login
I imagine that the neurological circuits underlying these processes are governed by both 2d spacing maps with their brains as…
to reduce the impact of car accidents, it may be possible to study the force diverting physics of cockroaches to…
you see this type of head-bobbing stability in many avian creatures related to pigeons like chickens. the head ability to…
not like they taught horses how to run! this is an example of convergent evolution where both sea creatures and…
The brain functions in a similar way with neuronal connections. our brains are able to utilize the multiplicity of connections…